Dataset for Spectroscopic, Structural and Dynamic Analysis of Human Fe(II)/2OG-Dependent Dioxygenase ALKBH3

Fe(II)/2OG-dependent dioxygenases of the AlkB family catalyze a direct removal alkylated damages in course DNA and RNA repair. A human homolog E. coli ALKBH3 protein is able to hydroxylate N1-methyladenine, N3-methylcytosine, N1-methylguanine single-stranded RNA. Due its contribution an antitumor drug resistance, this enzyme considered promising therapeutic target. The elucidation ALKBH3’s structural peculiarities important establish detailed mechanism damaged recognition processing, as well development specific inhibitors. This work presents new data on wild type four mutant forms (Y143F, Y143A, L177A, H191A) obtained by circular dichroism (CD) spectroscopy. dataset includes CD spectra proteins measured at different temperatures 3D visualization ALKBH3–DNA complex where mutated amino acid residues are marked. These results show how substitution key acids influences secondary structure content protein.